"Linking volatile metabolites from bacterial pathogens to exhaled breat" by P Hansani Karunarathne, Christopher Bridges et al.
 

Linking volatile metabolites from bacterial pathogens to exhaled breath condensate of people with cystic fibrosis.

Document Type

Article

Publication Date

2-2025

Publication Title

Microbiology (Reading)

Abstract

Obtaining sputum samples from people with cystic fibrosis (pwCF) for microbiology has become challenging due to the positive clinical effects of the cystic fibrosis transmembrane conductance regulator modulator therapy, elexacaftor-tezacaftor-ivacaftor (ETI). Although ETI improves lung function and reduces sputum production, recent data shows that bacterial pathogens persist, making continued monitoring of infection important. As an alternative to sputum sampling, this study developed a non-invasive technique called 'Cough Breath' (CB) to identify volatile organic compounds (VOCs) in exhaled breath condensate (EBC) and link them to cystic fibrosis (CF) bacterial pathogens using purge and trap GC-MS. The CB culturing approach was able to isolate pathogens from expectorated particulates simultaneously with EBC collection; however, culturing positivity was low, with 6% of samples collected (

Volume

171

Issue

2

DOI

10.1099/mic.0.001536

ISSN

1465-2080

PubMed ID

39976612

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