Exploring the role of Müller cells-derived exosomes in diabetic retinopathy.
Document Type
Article
Publication Date
7-2024
Publication Title
Microvascular research
Abstract
Exosomes are nanosized vesicles that have been reported as cargo-delivering vehicles between cells. Müller cells play a crucial role in the pathogenesis of diabetic retinopathy (DR). Activated Müller cells in the diabetic retina mediate disruption of barrier integrity and neovascularization. Endothelial cells constitute the inner blood-retinal barrier (BRB). Herein, we aim to evaluate the effect of Müller cell-derived exosomes on endothelial cell viability and barrier function under normal and hyperglycemic conditions. Müller cell-derived exosomes were isolated and characterized using Western blotting, nanoparticle tracking, and electron microscopy. The uptake of Müller cells-derived exosomes by the human retinal endothelial cells (HRECs) was monitored by labeling exosomes with PKH67. Endothelial cell vitality after treatment by exosomes under normo- and hypoglycemic conditions was checked by MTT assay and Western blot for apoptotic proteins. The barrier function of HRECs was evaluated by analysis of ZO-1 and transcellular electrical resistance (TER) using ECIS. Additionally, intracellular Ca
Volume
154
First Page
104695
Recommended Citation
Gad MS, Elsherbiny NM, El-Bassouny DR, Omar NM, Mahmoud SM, Al-Shabrawey M, et al Exploring the role of müller cells-derived exosomes in diabetic retinopathy. Microvasc Res. 2024 Jul;154:104695. doi: 10.1016/j.mvr.2024.104695. PMID: 38723843
DOI
10.1016/j.mvr.2024.104695
ISSN
1095-9319
PubMed ID
38723843
