Archives of Pathology & Laboratory Medicine
Context: Whole slide imaging (WSI) systems can provide multiple users with concurrent access to slides without the need to be physically present at the same location. However, the cost of an all-in-one WSI system can be prohibitive for programs that do not already use WSI systems at a clinical scale. Here, we delineate a simple, inexpensive WSI system that is easy to implement and meets the need of a resident teaching environment.
Design: A digital camera (Basler acA2500, Mannheim, Germany) is mounted to a brightfield microscope with a 0.63× C-mount and connected to a computer with the manual WSI program (Micro-Visioneer, Esslingen, Germany). Areas of interest on the slide are manually scanned, and the program stitches these images together to create an SVS file. We explored 2 possible access configurations: Web server based and intranet based (Figure 3.77).
Results: A collection of slides were manually scanned as described above. To assess the quality of these images, the slides were scanned a second time with an Aperio AT2. Both resulting sets of images were then compared to one another and the original glass slides to ensure suitable concordance of the images.
Conclusions: Our proposed solution for low-cost WSI for teaching and teleconsultation avoids the high upfront cost of an all-in-one packaged solution. High-quality whole slide images comparable to those generated by an Aperio scanner can be easily produced and accessed by multiple viewers simultaneously. Our proposed access solutions are similarly low cost, using open-source software and preexisting organizational network drives
Thorburn C, Deebajah M, Zhao K, Qu Z. A cost-conscious whole slide imaging system for teaching and teleconsultation in pathology. Arch Pathol Lab Med. 2022 Sep;146(9):e154. doi:10.5858/arpa.2022-0231-AB.