Document Type
Conference Proceeding
Publication Date
10-2022
Publication Title
Histopathology
Abstract
Background:Paneth cells with features ofneuroendocrine differentiation can be seen inmultiple organs and various tumours. Paneth cell-likegranules in clear cell renal cell carcinomas (RCC)have previously been reported, but are found noexpression of neuroendocrine markers. Given thatclear cell RCC are rich in phagocytic factors, wehypothesized that the Paneth cell-like granules inclear cell RCC were associated with lysosomes.Methods:In this study, 72 different renal tumoursincluded 42 clear cell RCC, 16 papillary RCC, 5 clearcell papillary RCC, 6 chromophobe RCC, 2 urothelialcarcinomas and one unclassified RCC. All tumourswere evaluated for the Paneth cell-like granules onhaematoxylin & eosin stained sections. In addition,PAS-D staining, immunohistochemical stains andelectron microscopy were conducted.Results:The Paneth cell-like granules were found in19% (8 out of 42) clear cell RCC, but not in othertypes of renal tumours. The granules stainedpositively on PAS-D stained section and were alsoimmunochemically positive for CD68 and lysozyme,two protein markers of lysosomes. Electronmicroscopy revealed that the Paneth cell-likegranules were smooth ball-shaped structures in thecytoplasm, ranging from 800 to 2400 nm in size,and best correlated with lysosomes.Conclusions:The Paneth cell-like granules in clearcell RCC are expanded lysosomes, which can be usedas a unique feature for confirming pathologicdiagnosis of clear cell RCC.Ó2021 The authors.Ó2021 John Wiley & Sons Ltd.Histopathology2022,81(Suppl. S1), 186–194. DOI: Background:Paneth cells with features ofneuroendocrine differentiation can be seen inmultiple organs and various tumours. Paneth cell-likegranules in clear cell renal cell carcinomas (RCC)have previously been reported, but are found noexpression of neuroendocrine markers. Given thatclear cell RCC are rich in phagocytic factors, wehypothesized that the Paneth cell-like granules inclear cell RCC were associated with lysosomes.Methods:In this study, 72 different renal tumoursincluded 42 clear cell RCC, 16 papillary RCC, 5 clearcell papillary RCC, 6 chromophobe RCC, 2 urothelialcarcinomas and one unclassified RCC. All tumourswere evaluated for the Paneth cell-like granules onhaematoxylin & eosin stained sections. In addition,PAS-D staining, immunohistochemical stains andelectron microscopy were conducted.Results:The Paneth cell-like granules were found in19% (8 out of 42) clear cell RCC, but not in othertypes of renal tumours. The granules stainedpositively on PAS-D stained section and were alsoimmunochemically positive for CD68 and lysozyme,two protein markers of lysosomes. Electronmicroscopy revealed that the Paneth cell-likegranules were smooth ball-shaped structures in thecytoplasm, ranging from 800 to 2400 nm in size,and best correlated with lysosomes.Conclusions:The Paneth cell-like granules in clearcell RCC are expanded lysosomes, which can be usedas a unique feature for confirming pathologicdiagnosis of clear cell RCC.
Volume
80
Issue
Suppl. 1
First Page
186
Last Page
186
Recommended Citation
Zhang PL. Paneth cell-like granules in clear cell renal cell carcinomas are CD68 and lysozyme positive lysosomes. Histopathology. 2022 Oct; 80(Suppl 1):186.
DOI
10.1111/his.14762