Title

Nondestructive, Indirect Assessment of the Biomechanical Properties of the Rat Intervertebral Disc Using Contrast-Enhanced μCT.

Document Type

Article

Publication Date

7-1-2018

Abstract

Mechanical characterization of the intervertebral disc involves labor-intensive and destructive experimental methodology. Contrast-enhanced micro-computed tomography is a nondestructive imaging modality for high-resolution visualization and glycosaminoglycan quantification of cartilaginous tissues. The purpose of this study was to determine whether anionic and cationic contrast-enhanced micro-computed tomography of the intervertebral disc can be used to indirectly assess disc mechanical properties in an ex vivo model of disc degeneration. L3/L4 motion segments were dissected from female Lewis rats. To deplete glycosaminoglycan, samples were treated with 0 U/ml (Control) or 5 U/ml papain. Contrast-enhanced micro-computed tomography was performed following incubation in 40% Hexabrix (anionic) or 30 mg I/ml CA

Comments

42nd Annual Meeting of the Orthopaedic Research Society, San Diego, CA. March 19-22, 2017.

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