Nondestructive, Indirect Assessment of the Biomechanical Properties of the Rat Intervertebral Disc Using Contrast-Enhanced μCT.
Mechanical characterization of the intervertebral disc involves labor-intensive and destructive experimental methodology. Contrast-enhanced micro-computed tomography is a nondestructive imaging modality for high-resolution visualization and glycosaminoglycan quantification of cartilaginous tissues. The purpose of this study was to determine whether anionic and cationic contrast-enhanced micro-computed tomography of the intervertebral disc can be used to indirectly assess disc mechanical properties in an ex vivo model of disc degeneration. L3/L4 motion segments were dissected from female Lewis rats. To deplete glycosaminoglycan, samples were treated with 0 U/ml (Control) or 5 U/ml papain. Contrast-enhanced micro-computed tomography was performed following incubation in 40% Hexabrix (anionic) or 30 mg I/ml CA
Newton MD, Hartner SE, Gawronski K, Davenport EJ, Timmons SC, Baker KC, Maerz T. Nondestructive, indirect assessment of the biomechanical properties of the rat intervertebral disc using contrast-enhanced μCT. J Orthop Res. 2018 Jul;36(7):2030-2038. doi: 10.1002/jor.23850. Epub 2018 Feb 7. PubMed PMID: 29314237.