Nondestructive, Indirect Assessment of the Biomechanical Properties of the Rat Intervertebral Disc Using Contrast-Enhanced μCT.

Authors

Michael D Newton, Beaumont Health
Samantha E Hartner, Beaumont Health
Karissa Gawronski, Beaumont Health
Tristan Maerz, Beaumont Health

Document Type

Article

Publication Date

7-1-2018

Abstract

Mechanical characterization of the intervertebral disc involves labor-intensive and destructive experimental methodology. Contrast-enhanced micro-computed tomography is a nondestructive imaging modality for high-resolution visualization and glycosaminoglycan quantification of cartilaginous tissues. The purpose of this study was to determine whether anionic and cationic contrast-enhanced micro-computed tomography of the intervertebral disc can be used to indirectly assess disc mechanical properties in an ex vivo model of disc degeneration. L3/L4 motion segments were dissected from female Lewis rats. To deplete glycosaminoglycan, samples were treated with 0 U/ml (Control) or 5 U/ml papain. Contrast-enhanced micro-computed tomography was performed following incubation in 40% Hexabrix (anionic) or 30 mg I/ml CA

Volume

36

Issue

7

First Page

2030

Comments

42nd Annual Meeting of the Orthopaedic Research Society, San Diego, CA. March 19-22, 2017.

Last Page

2038

Recommended Citation

Newton MD, Hartner SE, Gawronski K, Davenport EJ, Timmons SC, Baker KC, Maerz T. Nondestructive, indirect assessment of the biomechanical properties of the rat intervertebral disc using contrast-enhanced μCT. J Orthop Res. 2018 Jul;36(7):2030-2038. doi: 10.1002/jor.23850. Epub 2018 Feb 7. PubMed PMID: 29314237.

ISSN

1554-527X

PubMed ID

29314237