Amelioration of mucositis in proton therapy of fanconi anemia fanca-/- Mice by JP4-039
Document Type
Article
Publication Date
1-1-2019
Publication Title
In Vivo
Abstract
© 2019 International Institute of Anticancer Research. All rights reserved. Background/Aim: We tested JP4-039, a GS-nitroxide radiation damage mitigator in proton therapy of Fanconi anemia (FA) mice. Materials and Methods: Fanca-/- and Fanca+/+ bone marrow stromal cells were pre-treated with JP4-039 and irradiated with either protons or photons (0-10 GyRBE) followed by clonogenic survival and β-Galactosidase senescence analysis. Fanca-/- and Fanca+/+ mice were pretreated with JP4-039 for 10 min prior to oropharyngeal irradiation with either protons or photons (0 or 30 GyRBE) followed by sacrifice and measurement of oral cavity ulceration, distant hematopoietic suppression, and real-time polymerase chain reaction analysis. Results: JP4-039 reduced oral cavity ulceration in Fanca-/-mice, transcripts Nfkb, Ap1, Sp1, and Nrf2, and proton therapy induced distant marrow suppression. Conclusion: JP4-039 protected Fanca-/- and Fanca+/+ cells and mouse oral cavity from both proton and photon radiation.
Volume
33
Issue
6
First Page
1757
Last Page
1766
Recommended Citation
Quinn TJ, Ding X, Li X, Wilson GD, Buelow K, Sivananthan A, Thermozier S, Henderson A, Epperly MW, Franicola D, Wipf P, Greenberger JS, Stevens CW, Kabolizadeh P. Amelioration of Mucositis in Proton Therapy of Fanconi Anemia Fanca-/- Mice by JP4-039. In Vivo. 2019 Nov-Dec;33(6):1757-1766. doi: 10.21873/invivo.11666. PMID: 31662500; PMCID: PMC6899135.
DOI
10.21873/invivo.11666
ISSN
0258851X
PubMed ID
31662500