Functional Characterization of Small Alarmone Synthetase and Small Alarmone Hydrolase Proteins from Treponema denticola.
The stringent response enables bacteria to survive nutrient starvation, antibiotic challenge, and other threats to cellular survival. Two alarmone (magic spot) second messengers, guanosine pentaphosphate (pppGpp) and guanosine tetraphosphate (ppGpp), which are synthesized by RelA/SpoT homologue (RSH) proteins, play central roles in the stringent response. The pathogenic oral spirochete bacterium Treponema denticola lacks a long-RSH homologue but encodes putative small alarmone synthetase (Tde-SAS, TDE1711) and small alarmone hydrolase (Tde-SAH, TDE1690) proteins. Here, we characterize the respective
Wang M, Tang NY, Xie S, Watt RM. Functional characterization of small alarmone synthetase and small alarmone hydrolase proteins from treponema denticola. Microbiol Spectr. 2023 Jun 8:e0510022. doi: 10.1128/spectrum.05100-22. Epub ahead of print. PMID: 37289081.